K-Ras-ERK1/2 accelerates lung cancer cell development via mediating H3 K18ac through the MDM2-GCN5-SIRT7 axis

作者：Ziming Cheng, Xiufeng Li, Shizhen Hou, Yubing Wu, Yi Sun, Bing Liu · 发表于：Pharmaceutical Biology · 年份：2019 · DOI：10.1080/13880209.2019.1672756 · 被引用次数：11 · 研究领域：RNA Interference and Gene Delivery、CRISPR and Genetic Engineering、Sirtuins and Resveratrol in Medicine

Context: H3K18ac is linked to gene expression and DNA damage. Nevertheless, whether H3K18ac participates in regulating Ras-ERK1/2-affected lung cancer cell phenotypes remains unclear.Objective: We explored the effects of H3K18ac on Ras-ERK1/2-affected lung cancer cell phenotypes.Material and methods: NCI-H2126 cells were transfected with, pEGFP-K-RasWT and pEGFP-K-RasG12V/T35S plasmids for 48 h, and transfection with pEGFP-N1 served as a blank control. Then H3K18ac and AKT and ERK1/2 pathways-associated factors were examined. Different amounts of the H3K18Q (0.5, 1, and 2 μg) plasmids and RasG12V/T35S were co-transfected into NCI-H2126 cells, cell viability, cell colonies and migration were analyzed for exploring the biological functions of H3K18ac in NCI-H2126 cells. The ERK1/2 pathway downstream factors were detected by RT-PCR and ChIP assays. The regulatory functions of SIRT7, GCN5 and MDM2 in Ras-ERK1/2-regulated H3K18ac expression were finally uncovered.Results: RasG12V/T35S transfection decreased the expression of H3K18ac about 2.5 times compared with the pEGFP-N1 transfection group, and activated ERK1/2 and AKT pathways. Moreover, H3K18ac reduced cell viability, colonies, migration, and altered ERK1/2 downstream transcription in NCI-H2126 cells. Additionally, SIRT7 knockdown increased H3K18ac expression and repressed cell viability, migration and the percentage of cells in S phase by about 50% compared to the control group, as well as changed ERK1/2 downstream factor e...