Etchable SERS nanosensor for accurate pH and hydrogen peroxide sensing in living cells

作者：Lu Bai, Xiaojie Wang, Kaifu Zhang, Xiaoyue Tan, Yuying Zhang, Wei Xie · 发表于：Chemical Communications · 年份：2019 · DOI：10.1039/c9cc06485k · 被引用次数：37 · 研究领域：Advanced Nanomaterials in Catalysis、Electrochemical sensors and biosensors、Advanced biosensing and bioanalysis techniques

No methodology has been built to distinguish intracellular SERS nanosensors from cell outer membrane bound-ones. Here we propose a "turning off" strategy by combining etchable SERS nanosensors with a non-permeable etchant. The SERS signals outside the living cells can be rapidly removed, leaving only the internalized nanosensors for imaging and thereby allowing accurate intracellular pH and H2O2 sensing.