Biocontrol of Citrus Canker Disease Caused by Xanthomonas citri subsp. citri Using an Endophytic Bacillus thuringiensis

作者：Md. Nurul Islam, Md. Sarafat Ali, Seong-Jin Choi, Jae-Wook Hyun, Kwang‐Hyun Baek · 发表于：The Plant Pathology Journal · 年份：2019 · DOI：10.5423/ppj.oa.03.2019.0060 · 被引用次数：63 · 研究领域：Plant-Microbe Interactions and Immunity、Plant Pathogenic Bacteria Studies、Plant Pathogens and Fungal Diseases

Citrus canker is a devastating disease of citrus caused by Xanthomonas citri subsp.citri (Xcc).A total of 134 endophytic bacteria were isolated from various gymnospermic and angiospermic plants.They were screened for their antagonistic activities against three wild-type and six streptomycin-resistant Xcc strains.TbL-22 and TbL-26, both later identified as Bacillus thuringiensis, inhibited all the wild and resistant Xcc strains.TbL-22 exerted the highest antagonistic activity against XccW3 and XccM6 with inhibition zones of 20.64 ± 0.69 and 19.91 ± 0.87 mm, respectively.Similarly ethyl acetate extract of TbL-22 showed highest inhibition zones 15.31 ± 2.08 and 19.37 ± 3.17 mm against XccW3 and XccM6, respectively.TbL-22 reduced canker incidence on infected leaves by 64.05% relative to positive controls.Scanning electron microscopy revealed that the cell membranes of Xcc treated with ethyl acetate extract of TbL-22 were ruptured, lysed, and swollen.B. thuringiensis TbL-22 can effectively and sustainably controls streptomycin-resistant citrus canker.