S‐Click Reaction for Isotropic Orientation of Oxidases on Electrodes to Promote Electron Transfer at Low Potentials

作者：Lin Xia, Ming‐Jie Han, Lu Zhou, Aiping Huang, Zhao-ya Yang, Tianyuan Wang, Fahui Li, Lu Yu, Changlin Tian, Zhongsheng Zang, Qing‐Zheng Yang, Chenli Liu, Wen‐Xu Hong, Yi Lu, Lital Alfonta, Jiangyun Wang · 发表于：Angewandte Chemie International Edition · 年份：2019 · DOI：10.1002/anie.201909203 · 被引用次数：24 · 研究领域：Electrochemical sensors and biosensors、Conducting polymers and applications、Electrochemical Analysis and Applications

Electrochemical sensors are essential for point-of-care testing (POCT) and wearable sensing devices. Establishing an efficient electron transfer route between redox enzymes and electrodes is key for converting enzyme-catalyzed reactions into electrochemical signals, and for the development of robust, sensitive, and selective biosensors. We demonstrate that the site-specific incorporation of a novel synthetic amino acid (2-amino-3-(4-mercaptophenyl)propanoic acid) into redox enzymes, followed by an S-click reaction to wire the enzyme to the electrode, facilitates electron transfer. The fabricated biosensor demonstrated real-time and selective monitoring of tryptophan (Trp) in blood and sweat samples, with a linear range of 0.02-0.8 mm. Further developments along this route may result in dramatic expansion of portable electrochemical sensors for diverse health-determination molecules.