Nanosilver-incorporated halloysite nanotubes/gelatin methacrylate hybrid hydrogel with osteoimmunomodulatory and antibacterial activity for bone regeneration

作者：Qianmin Ou, Keqing Huang, Chuanqiang Fu, Chunlin Huang, Yifei Fang, Zhipeng Gu, Jun Wu, Yan Wang · 发表于：Chemical Engineering Journal · 年份：2019 · DOI：10.1016/j.cej.2019.123019 · 被引用次数：123 · 研究领域：Oral microbiology and periodontitis research、Periodontal Regeneration and Treatments、Oral and gingival health research

After a biomaterial is implanted in a bone defect area, the immune response and bacterial infection affect the success of bone regeneration. In this study, we describe the development of a promising therapeutic approach to accelerate bone regeneration via combining osteoimmunomodulatory and antibacterial activities. Herein, we fabricated a nanosilver/halloysite nanotubes/gelatin methacrylate (nAg/HNTs/GelMA) hybrid hydrogel and evaluated its osteoimmunomodulatory and antibacterial properties in vitro and in vivo. The nAg/HNTs/GelMA hybrid hydrogel had good biocompatibility with human periodontal ligament stem cells (hPDLSCs) and macrophages. Moreover, the nAg/HNTs/GelMA hybrid hydrogel modulated inflammatory cytokines secreted by macrophages and enhanced the osteogenic differentiation of hPDLSCs in an inflammatory environment. In addition, nAg/HNTs/GelMA hybrid hydrogel inhibited the growth of Gram-positive and Gram-negative bacteria in vitro and in vivo. Compared with HNTs/GelMA hydrogel, the nAg/HNTs/GelMA hybrid hydrogel better modulated the osteoimmune microenvironment and eliminated bacterial infection. Thus, this hybrid hydrogel combining osteoimmunomodulatory with antibacterial activities is a promising biomaterial for bone regeneration in defect areas.