Catalytic inactivation of influenza virus by iron oxide nanozyme

作者：Tao Qin, Ruonan Ma, Yinyan Yin, Xinyu Miao, Sujuan Chen, Kelong Fan, Juqun Xi, Qi Liu, Yunhao Gu, Yuncong Yin, Jiao Hu, Xiufan Liu, Daxin Peng, Lizeng Gao · 发表于：Theranostics · 年份：2019 · DOI：10.7150/thno.35826 · 被引用次数：139 · 研究领域：Advanced Nanomaterials in Catalysis、Mosquito-borne diseases and control、Extracellular vesicles in disease

Influenza poses a severe threat to human health in the world. However, developing a universal anti-viral strategy has remained challenging due to the presence of diverse subtypes as well as its high mutation rate, resulting in antigenic shift and drift. Here we developed an antiviral strategy using iron oxide nanozymes (IONzymes) to target the lipid envelope of the influenza virus. Methods: We evaluated the antiviral activities of our IONzymes using a hemagglutination assay, together with a 50% tissue culture infectious doses (TCID 50 ) method. Lipid peroxidation of the viral envelope was analyzed using a maleic dialdehyde (MDA) assay and transmission electron microscopy (TEM). The neighboring viral proteins were detected by western blotting. Results: We show that IONzymes induce envelope lipid peroxidation and destroy the integrity of neighboring proteins, including hemagglutinin, neuraminidase, and matrix protein 1, causing the inactivation of influenza A viruses (IAVs). Furthermore, we show that our IONzymes possess a broad-spectrum antiviral activity on 12 subtypes of IAVs (H1~H12). Lastly, we demonstrate that applying IONzymes to a facemask improves the ability of virus protection against 3 important subtypes that pose a threat to human, including H1N1, H5N1, and H7N9 subtype. Conclusion: Together, our results clearly demonstrate that IONzymes can catalyze lipid peroxidation of the viral lipid envelope to inactivate enveloped viruses and provide protection from viral tra...