Research of photosynthesis and genomewide resequencing on a yellow‐leaf Lotus japonicus mutant induced by carbon ion beam irradiation

作者：Tao Cui, Shanwei Luo, Yan Du, Lixia Yu, Jiang-Yan Yang, Wenjian Li, Chen Xia, Xin Li, Jie Wang, Libin Zhou · 发表于：Grassland Science · 年份：2018 · DOI：10.1111/grs.12216 · 被引用次数：31 · 研究领域：Plant Genetic and Mutation Studies、Photosynthetic Processes and Mechanisms、Plant tissue culture and regeneration

Abstract Carbon ion beam is an advanced physical mutagen that is used for mutation breeding and functional genomics research. Using carbon ion beams, a stable Lotus japonicus mutant C16 which showed yellow leaves and reduced height compared with the laboratory wild type was obtained in our previous study. Yellow‐leaf mutation is a desirable characteristic for studying photosynthesis. The photosynthetic‐related physiological indexes, including the content of chlorophylls and the ratio of variable fluorescence ( F v ) over the maximum fluorescence value ( F m ) (maximal photochemical efficiency of photosystem II in the dark) of leaves between laboratory wild type and C16, were investigated. C16 showed lower amount of chlorophylls than that of laboratory wild type, as well as a relatively photosynthetic capacity. Meanwhile, to comprehend the molecular mechanism underlying the mutant, genomewide resequencing was performed to identify the mutational site responsible for the yellow‐leaf phenotype causing the weaker photosynthetic capacity. Ninety‐five single‐base substitutions and 17 small insertions and deletions were found in C16 mutant. Among those, by association analysis of functional annotation and mutant phenotype, T>G SBS in chromosome 5 located in Lj5g3v1669360.1 which encodes arabinogalactan protein was supposed as candidate mutation sites. Quantitative real‐time polymerase chain reaction analysis indicated that the expression level of Lj5g3v1669360.1 in C16 was signif...