Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches

作者：Wenjing Liu, Yourong Guo, Kaizhe Wang, Xingfei Zhou, Ying Wang, Junhong Lü, Zhifeng Shao, Jun Hu, Daniel M. Czajkowsky, Bin Li · 发表于：Nanoscale · 年份：2019 · DOI：10.1039/c9nr05234h · 被引用次数：19 · 研究领域：Force Microscopy Techniques and Applications、Advanced biosensing and bioanalysis techniques、Molecular Junctions and Nanostructures

Atomic force microscopy-based single-molecule-force spectroscopy is limited by low throughput. We introduce addressable DNA origami to study multiple target molecules. Six target DNAs that differed by only a single base-pair mismatch were clearly differentiated a rupture force of only 4 pN.