The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China

作者：Zhenshan Deng, Xiaodong Liu, Baocheng Zhang, Shuo Jiao, Xiangying Qi, Zhi-Hong Sun, Xiaolong He, Yuzhen Liu, Jing Li, Kai-Kai Chen, Zhanxi Lin, Yingying Jiang · 发表于：Microorganisms · 年份：2019 · DOI：10.3390/microorganisms7090332 · 被引用次数：14 · 研究领域：Plant Pathogens and Fungal Diseases、Plant and fungal interactions、Mycorrhizal Fungi and Plant Interactions

Pennisetum sinese is a good forage grass with high biomass production and crude proteins. However, little is known about the endophytic fungi diversity of P. sinese, which might play an important role in the plant’s growth and biomass production. Here, we used high throughput sequencing of the Internal Transcribed Spacer (ITS) sequences based on primers ITS5-1737 and ITS2-2043R to investigate the endophytic fungi diversity of P. sinese roots at the maturity stage, as collected from four provinces (Shaanxi province, SX; Fujian province, FJ; the Xinjiang Uyghur autonomous prefecture, XJ and Inner Mongolia, including sand (NS) and saline-alkali land (NY), China). The ITS sequences were processed using QIIME and R software. A total of 374,875 effective tags were obtained, and 708 operational taxonomic units (OTUs) were yielded with 97% identity in the five samples. Ascomycota and Basidiomycota were the two dominant phyla in the five samples, and the genera Khuskia and Heydenia were the most abundant in the FJ and XJ samples, respectively, while the most abundant tags in the other three samples could not be annotated at the genus level. In addition, our study revealed that the FJ sample possessed the highest OTU numbers (242) and the NS sample had the lowest (86). Moreover, only 22 OTUs were present in all samples simultaneously. The beta diversity analysis suggested a division of two endophytic fungi groups: the FJ sample from the south of China and the other four samples from no...