LOV-based reporters for fluorescence imaging

作者：Anthony M. Buckley, Jan Petersen, Andrew J. Roe, Gill R. Douce, John McDougall Christie · 发表于：Current Opinion in Chemical Biology · 年份：2015 · DOI：10.1016/j.cbpa.2015.05.011 · 被引用次数：125 · 研究领域：Light effects on plants、Advanced Fluorescence Microscopy Techniques、Photosynthetic Processes and Mechanisms

Chromophore-binding domains from plant and bacterial photoreceptor proteins have recently gathered increasing attention as new sources of genetically encoded fluorescent proteins (FPs). In particular, FPs based on the flavin-binding LOV (light, oxygen, or voltage sensing) domain offer advantages over green fluorescent protein (GFP) owing to their smaller size, pH and thermal stability, utility under anaerobic conditions and their ability to generate reactive oxygen species. This review focuses on the potential applications of this emerging class of fluorescent reporters, discusses the advantages and limitations of LOV-based FPs, whilst offering insights regarding the further development of this technology for bioimaging and photodynamic therapy.