[Effects of Cellular Density on the Induction of Suspension Globe of Ovarian Cancer Stem Cells].

作者：Mianzhi Chen, Lan Zhong, Dongmei Wei, Xiaoyu Niu · 发表于：PubMed · 年份：2017 · 被引用次数：2 · 研究领域：Cancer Cells and Metastasis、Cancer Research and Treatments、Cancer Mechanisms and Therapy

OBJECTIVE: To determine the effect of cellular density on the separation and identification of cancer stem cells from human ovarian clear cell carcinoma cell line ES-2 and adenocarcinoma cell line A2780. METHODS: ES-2 and A2780 cells were cultured with human recombinant epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) and bovine serum albumin and insulin in serum free medium. The cancer stem cells were obtained through serial passages. Changes in cell morphology,expressions of surface marker CD133 and CD44,and soft AGAR clone forming in the stem cells were examined under different cell density,either in serum-supplemented medium (SSM group) or in serum free medium (SFM group). RESULTS: . CONCLUSION: for A2780,respectively.