Antifungal, Plant Growth-Promoting, and Genomic Properties of an Endophytic Actinobacterium Streptomyces sp. NEAU-S7GS2

作者：Dongli Liu, Rui Yi Yan, Yansong Fu, Xiangjing Wang, Ji Zhang, Wensheng Xiang · 发表于：Frontiers in Microbiology · 年份：2019 · DOI：10.3389/fmicb.2019.02077 · 被引用次数：122 · 研究领域：Plant-Microbe Interactions and Immunity、Plant Pathogens and Fungal Diseases、Plant Disease Resistance and Genetics

Diseases caused by Sclerotinia sclerotiorum have caused severe losses of many economically important crops worldwide. Due to the long term persistence of sclerotia in the soil and the production of air-borne ascospores, the synthetic fungicides play limited role to control the diseases. The application of antagonistic microorganisms can effectively reduce the number of sclerotia and eventually eradicate S. sclerotiorum from soil, therefore considerable interest has been focused on biological control. Streptomyces sp. NEAU-S7GS2 was isolated from the root of Glycine max and its rhizosphere soil. It showed significantly inhibitory activity against the mycelial growth of S. sclerotiorum with an inhitory ratio of 99.1% and completely inhibited the sclerotia germination. In the pot experiment, compared to the control, the application of NEAU-S7GS2 not only demonstrated excellent potential to control sclerotinia stem rot of soybean with 77% and 38% decrease in disease incidence and disease index, respectively, but could promote the growth of soybean. The light microscope and scanning electron microscopy showed that co-culture of NEAU-S7GS2 with S. sclerotiorum on potato dextrose agar could lead to contorted and fragmented mycelia of S. sclerotiorum, which was associated with the secretion of hydrolytic glucanse and cellulase and the production of active secondary metabolites by NEAU-S7GS2. The plant growth promoting activity of NEAU-S7GS2 was related to the solubilization of inorga...