FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum

作者：Xiangjiu Kong, Hao Zhang, Xiao‐Liang Wang, Théo van der Lee, Cees Waalwijk, Anne van Diepeningen, Balázs Brankovics, Jin Xu, Jingsheng Xu, Wanquan Chen, Jie Feng · 发表于：Frontiers in Microbiology · 年份：2019 · DOI：10.3389/fmicb.2019.02088 · 被引用次数：24 · 研究领域：Plant Disease Resistance and Genetics、Mycotoxins in Agriculture and Food、Plant and fungal interactions

Peroxisomes are involved in a wide range of important cellular functions.. We studied Here, the role of the peroxisomal membrane protein PEX3 proposed docking receptor of the peroxisome, PEX3 in the plant-pathogen and mycotoxin producer Fusarium graminearum was studied. To study the function of PEX3 using knock-out and complementation strains. were constructed by gene replacements. As import of proteins depends on PTS1 and PTS2 signatures , To fluorescently label peroxisomes punctate structures, GFP and RFP fusions with the PTS1 and PTS2 localization signal were transformed into the wild type PH-1, and ΔFgPex3 knock-out and complementation strains. The GFP and RFP transformants in theDeletion of ΔFgPex3 background showed a diffuse fluorescence pattern across the cytoplasm suggesting the absence of peroxisomes.blocked the import of peroxisome matrix proteins through both PTS1 and PTS2 signaling pathway. The ΔFgPex3 strain showed a minor , non-significant reduction in growth on various sugar carbon sources. In contrast, deletion of However, FgPex3 did affect the fatty acid β-oxidation in F. graminearum and significantly reduced the utilization of fatty acids. Furthermore, the ΔFgPex3 knock-outmutant was sensitive to osmotic stressors as well as to cell wall-damaging agents. Reactive oxygen species (ROS) levels in the mutant had increased significantly, which may be linked to the reduced longevity of cultured strains. The mutant also showed reduced production of conidiospores, w...