Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics

作者：Shanshan Su, Jisheng Zhang, Yongxin Zhao, Lan Yu, Yong Wang, Yuchao Wang, Mingjia Bao, Yu Fu, Chunjiang Li, Xiaoli Zhang · 发表于：BMC Infectious Diseases · 年份：2020 · DOI：10.1186/s12879-020-05143-y · 被引用次数：28 · 研究领域：Antibiotic Resistance in Bacteria、Bacterial Identification and Susceptibility Testing、Infections and bacterial resistance

Abstract Background Carbapenem-resistant hypervirulent Klebsiella pneumoniae strains have recently come into existence worldwide; however, researchers in northeast China are not aware of their clinical features and molecular characteristics. Methods Here, the molecular and virulent characteristics of 44 carbapenem-resistant K. pneumoniae (CRKP) isolates collected from January 2015 to December 2017 were studied. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were carried out to define the clonal relatedness among the isolates. PCR and capsular serotyping of the virulence-associated genes, as well as biofilm formation and serum complement-mediated killing assays, were employed to determine the virulent potential. The genomic features and associated mobile genetic elements of JmsCRE57 were detected by whole genome sequencing. Results The only positive isolate was JmsCRE57, which belonged to the ST375 serotype K2 that expressed uge , mrkD , fimH , kpn , aerobactin and rmpA virulence-associated genes and showed strong biofilm formation and serum sensitivity. Sequencing results showed that the JmsCRE57 genome mainly consisted of a circular chromosome, three antimicrobial resistant plasmids and a virulent plasmid. The antimicrobial resistant plasmid expressing bla KPC-2 , bla CTX-M-15 , aph(3″)-Ib , aph(6)-Id , qnrB1 , aac(3)-IIa , aac(6′)-Ib-cr , bla OXA-1 , bla TEM-1B , catB4 , sul2 , dfrA14 and bla SHV-99 . The virulent plasmid belonged to the IncHI...