Interleukin‐8/β‐catenin mediates epithelial–mesenchymal transition in ameloblastoma

作者：Jie Zhang, Yanhui Wang, Cunhui Fan, Ximei Xiao, Qian Zhang, Tao Xu, Chunmiao Jiang · 发表于：Oral Diseases · 年份：2019 · DOI：10.1111/odi.13173 · 被引用次数：21 · 研究领域：Oral and Maxillofacial Pathology、Cancer Cells and Metastasis、Wnt/β-catenin signaling in development and cancer

OBJECTIVE: Epithelial-mesenchymal transition (EMT) is important in the tooth development and tumor invasion. We investigated the effect of interleukin-8 (IL-8) on the EMT process in primary-cultured ameloblastoma tumor cells (AM-P) and ameloblastoma immortalized tumor cells (AM-L) and its underlying mechanism. METHODS: IL-8 levels in ameloblastomas were detected by immunofluorescence staining and ELISA. AM-P cells and AM-L cells were stimulated with IL-8, and EMT transcription factors, total β-catenin and phosphorylated-β-catenin (p-β-catenin) levels were determined by Western blot analysis and immunofluorescence staining. β-catenin siRNA was used to knockdown β-catenin expression in AM-P cells and AM-L cells stimulated with IL-8. RESULTS: IL-8 was highly expressed in the solid ameloblastomas. IL-8 promoted the EMT process in ameloblastoma tumor cells in vitro, as evidenced by decreased E-cadherin and increased vimentin, twist and zeb1 levels. IL-8 also increased total β-catenin and p-β-catenin expression in ameloblastoma tumor cells, and β-catenin knockdown partially inhibited the EMT process in tumor cells, as evidenced by increased E-cadherin, and decreased vimentin and zeb1 levels. CONCLUSIONS: IL-8 could promote EMT in ameloblastoma tumor cells by activating β-catenin and its downstream transcription factor zeb1.