Ageing affects the proliferation and mineralization of rat dental pulp stem cells under inflammatory conditions

作者：Tingting Ning, J. Shao, X. Zhang, Xinghong Luo, Xiangyu Huang, Hansheng Wu, Shuaimei Xu, Buling Wu, Dandan Ma · 发表于：International Endodontic Journal · 年份：2019 · DOI：10.1111/iej.13205 · 被引用次数：26 · 研究领域：Mesenchymal stem cell research、Bone and Dental Protein Studies、Endodontics and Root Canal Treatments

Abstract Aim To comparatively evaluate changes in the proliferation and mineralization abilities of dental pulp stem cells (DPSCs) from juvenile and adult rats in a lipopolysaccharide (LPS)‐induced inflammatory microenvironment to provide a theoretical basis for the age‐related differences observed in DPSCs during repair of inflammatory injuries. Methodology DPSCs were isolated from juvenile (JDPSCs) and adult rats (ADPSCs), and senescence‐associated β‐galactosidase staining was used to compare senescence between JDPSCs and ADPSCs. Effects of LPS on JDPSCs and ADPSCs proliferation were investigated by cell counting kit‐8 assays and flow cytometry. Alizarin red staining, quantitative reverse transcription polymerase chain reaction and Western blot assay were used to examine the effects of LPS on mineralization‐related genes and proteins in JDPSCs and ADPSCs. Immunohistochemistry was used to compare interleukin‐1β (IL‐1β) and osteocalcin (OCN) expression in the pulpitis model. Unpaired Student's t ‐tests and one‐way anova were used for statistical analysis. Results DPSCs were isolated from juvenile and adult rat dental pulp tissues. At low concentrations (0.1–1 μg mL −1 ), LPS significantly promoted the proliferation of JDPSCs ( P < 0.01) and ADPSCs ( P < 0.01 or P < 0.05), with the effect being stronger in JDPSCs than in ADPSCs. In addition, mineralized nodules and the expression of mineralization‐related genes ( OCN , DSPP , ALP , BSP ) increased significantly after ...