Kaempferol attenuates imiquimod-induced psoriatic skin inflammation in a mouse model

作者：Chao-Shui Liu, Han Liu, Chuanjian Lu, Jingwen Deng, Yihe Yan, Huaichun Chen, Yuan-Zhong Wang, C-L Liang, Jianan Wei, Ling Han, Zhenhua Dai · 发表于：Clinical & Experimental Immunology · 年份：2019 · DOI：10.1111/cei.13363 · 被引用次数：74 · 研究领域：Psoriasis: Treatment and Pathogenesis、Dermatology and Skin Diseases、T-cell and B-cell Immunology

Summary Psoriasis is an immune-mediated inflammatory skin disease that mainly affects the skin barrier. Treatment for psoriasis mainly includes conventional immunosuppressive drugs. However, long-term treatment with global immunosuppressive agents may cause a variety of side effects, including nephrotoxicity and infections. Kaempferol, a natural flavonol present in various plants, is known to possess potent anti-inflammatory, anti-oxidant and anti-cancerous properties. However, it is unknown whether kaempferol is also anti-psoriatic. Here we established an imiquimod (IMQ)-induced psoriatic mouse model to explore the potential therapeutic effects of kaempferol on psoriatic skin lesions and inflammation. In this study, we demonstrated that treatment with kaempferol protected mice from developing psoriasis-like skin lesions induced by topical administration of IMQ. Kaempferol reduced CD3+ T cell infiltration and gene expression of major proinflammatory cytokines, including interleukin (IL)-6, IL-17A and tumor necrosis factor (TNF)-α, in the psoriatic skin lesion. It also down-regulated proinflammatory nuclear factor kappa B (NF-κB) signaling in the skin. The therapeutic effects were associated with a significant increase in CD4+forkhead box protein 3 (FoxP3)+ regulatory T cell (Treg) frequency in the spleen and lymph nodes as well as FoxP3-positive staining in the skin lesion. Conversely, depletion of CD4+CD25+ Tregs reversed the therapeutic effects of kaempferol on the skin les...