Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China

作者：Yan Bai, Xiying Huang, Xiangrui Zhou, Quanju Xiang, Ke Zhao, Xiumei Yu, Qiang Chen, Hao Jiang, Nyima Tashi, Xue Gao, Yunfu Gu · 发表于：PeerJ · 年份：2019 · DOI：10.7717/peerj.7356 · 被引用次数：13 · 研究领域：Microbial Community Ecology and Physiology、Polar Research and Ecology、Soil Carbon and Nitrogen Dynamics

Background The Hailuogou Glacier is located at the Gongga Mountain on the southeastern edge of the Tibetan Plateau, and has retreated continuously as a result of global warming. The retreat of the Hailuogou Glacier has left behind a primary succession along soil chronosequences. Hailuogou Glacier’s retreated area provides an excellent living environment for the colonization of microbes and plants, making it an ideal model to explore plant successions, microbial communities, and the interaction of plants and microbes during the colonization process. However, to date, the density of the nitrogen cycling microbial communities remain unknown, especially for denitrifiers in the primary succession of the Hailuogou Glacier. Therefore, we investigated the structural succession and its driving factors for denitrifying bacterial communities during the four successional stages (0, 20, 40, and 60 years). Methods The diversity, community composition, and abundance of nosZ -denitrifiers were determined using molecular tools, including terminal restriction fragment length polymorphism and quantitative polymerase chain reactions (qPCR). Results nosZ -denitrifiers were more abundant and diverse in soils from successional years 20–60 compared to 0–5 years, and was highest in Site3 (40 years). The denitrifying bacterial community composition was more complex in older soils (40–60 years) than in younger soils (≤20 years). The terminal restriction fragments (T-RFs) of Azospirillum (90 bp) and Rub...