Cynomorium Songaricum may protect against spermatogenic damage caused by cyclophosphamide in SD rats

作者：Xiaoxiao Han, Rongsheng Zhou, Zheng Wei, Xitao Wang, Shuyan Mao, Zhenbei Li, Lin Hao, Zhenduo Shi, Bo Chen, Zhiguo Zhang, Yijuan Cao, Dian‐Jun Yu, Kun Pang, Conghui Han · 发表于：Revista română de medicină de laborator · 年份：2019 · DOI：10.2478/rrlm-2019-0031 · 被引用次数：3 · 研究领域：Chemotherapy-induced organ toxicity mitigation、Reproductive Biology and Fertility、Sperm and Testicular Function

Abstract Aim: The aim of the study is to investigate the effect of Cynomorium songaricum (CS) on the damage caused by cyclophosphamide (CP) in SD rats. Methods: Rats with CP-induced oligoasthenospermia were treated with different concentration of CS. Testicle weight, epididymal sperm count (ESC), sperm motility, and serum testosterone were analyzed, and expression levels of Thy1, Oct4, PLZF, C-kit, and GDNF were detected in testis tissues. Transmission electron microscopy (TEM) was performed to observe the effect of CS on the spermatogenic damage by CP. Results: Compared with the CP group, there were significant differences in testicle weight, ESC, and sperm motility (p<0.05) observed in all concentrations of CS and CP+VitE groups (p<0.05). There were no significant differences in serum testosterone among the 6 groups (p>0.05). The qPCR results revealed a significant difference in Thy1, Oct4, PLZF and GDNF expression between the CP group and CS group (p <0.05), but there was no significant difference in C-kit between the two groups (p>0.05). The damage of CP was cured by CS observed under TEM. Conclusion: CS can increase sperm counts in the epididymis and improve sperm motility and has a therapeutic effect on the spermatogenic damage caused by CP in SD rats.