Thylakoid localized bestrophin-like proteins are essential for the CO 2 concentrating mechanism of Chlamydomonas reinhardtii

作者：Ananya Mukherjee, Chun Sing Lau, Charlotte E. Walker, Ashwani K. Rai, Camille I. Prejean, Gary F. Yates, Tom Z. Emrich‐Mills, Spencer G. Lemoine, David J. Vinyard, Luke C. M. Mackinder, James V. Moroney · 发表于：Proceedings of the National Academy of Sciences · 年份：2019 · DOI：10.1073/pnas.1909706116 · 被引用次数：136 · 研究领域：Photosynthetic Processes and Mechanisms、Algal biology and biofuel production、Photoreceptor and optogenetics research

Significance Models of the CO 2 concentrating mechanism (CCM) of green algae and diatoms postulate that chloroplast CO 2 is generated from HCO 3 − brought into the acidic thylakoid lumen and converted to CO 2 by specific thylakoid carbonic anhydrases. However, the identity of the transporter required for thylakoid HCO 3 − uptake has remained elusive. In this work, 3 bestrophin-like proteins, BST1–3, located on the thylakoid membrane have been found to be essential to the CCM of Chlamydomonas . Reduction in expression of BST1–3 markedly reduced the inorganic carbon affinity of the alga. These proteins are prime candidates to be thylakoid HCO 3 − transporters, a critical currently missing step of the CCM required for future engineering efforts of the Chlamydomonas CCM into plants to improve photosynthesis.