Linear Peptides Containing d -Leucine with Neuroprotective Activities from the Leech Whitmania pigra Whitman

作者：Ziyue Zhang, Nan Ma, Lijun Tao, Xue-Ying Gong, Wen‐Cai Ye, Lei Wang · 发表于：Journal of Natural Products · 年份：2019 · DOI：10.1021/acs.jnatprod.9b00322 · 被引用次数：17 · 研究领域：Leech Biology and Applications、Healthcare and Venom Research、Venomous Animal Envenomation and Studies

Three new linear peptides containing d -leucine, named whitmantides A–C ( 1 – 3 ), were isolated from the dried whole bodies of Whitmania pigra Whitman. Their structures with absolute configurations were elucidated by Edman degradation, mass spectrometry, Marfey’s analysis, and solid-phase synthesis. It is the first time that peptides containing d -amino acid in leeches were discovered. Compounds 1 – 3 displayed neuroprotective activities against oxygen–glucose deprivation/reperfusion injury on Neuro-2a cells. In addition, ex vivo serum stability tests showed that 1 – 3 were resistant to protease degradation.