Improving the Quality and Reproducibility of Flow Cytometry in the Lung. An Official American Thoracic Society Workshop Report

作者：Robert M. Tighe, Elizabeth F. Redente, Yen-Rei Andrea Yu, Susanne Herold, Anne I. Sperling, Jeffrey L. Curtis, Ryan C. Duggan, Suchitra Swaminathan, Hideki Nakano, William J. Zacharias, William J. Janssen, Christine M. Freeman, Ryan R. Brinkman, Benjamin David Singer, Claudia Jakubzick, Alexander V. Misharin · 发表于：American Journal of Respiratory Cell and Molecular Biology · 年份：2019 · DOI：10.1165/rcmb.2019-0191st · 被引用次数：71 · 研究领域：Neonatal Respiratory Health Research、Single-cell and spatial transcriptomics、Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis

Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.