Transport stress induces pig jejunum tissue oxidative damage and results in autophagy/mitophagy activation

作者：Yulong He, Zhan Sang, Yisha Zhuo, Xueyi Wang, Zeheng Guo, Lihua He, Cuiping Zeng, Hanchuan Dai · 发表于：Journal of Animal Physiology and Animal Nutrition · 年份：2019 · DOI：10.1111/jpn.13161 · 被引用次数：39 · 研究领域：Autophagy in Disease and Therapy、Ginger and Zingiberaceae research、Cannabis and Cannabinoid Research

Pig transportation is associated with intestinal oxidative stress and results in destruction of intestinal integrity. Autophagy has been contributed to maintain cell homeostasis under stresses. The purpose of this study was to evaluate the effects of transport stress on morphology, intestinal mucosal barrier and autophagy/mitophagy levels in pig jejunum. A total of 16 finishing pigs were randomly divided into two groups. The control group was directly transported to the slaughterhouse and rested for 24 hr. The experimental groups were transported for 5 hr and slaughtered immediately. The results showed that transportation induced obvious stress responses with morphological and histological damage in jejunum accompanying with an elevated level of malondialdehyde (MDA; p < .05), endotoxin (LPS; p < .05), lactic dehydrogenase (LDH; p < .05) and a decreased level of serum superoxide dismutase (SOD; p < .05). Also, hemeoxy genase 1 (HO-1; p < .01) as well as tight junction protein (claudin-1 [p < .001], occludin [p < .05] and zonula occludens 1 [ZO-1; p < 0.05]) levels were attenuated in jejunum tissue, and NADPH oxidase 1 (NOX1; p < .01) mRNA expression was up-regulated. Further research indicated that transport stress could induce autophagy through increasing microtubule-associated protein light chain 3 (LC3; p < .05) and autophagy-related gene 5 (ATG5; p < .01) levels and suppressing p62 expression. Additionally, transport stress increased the protein levels of PTEN-induced put...