Extension of the in vivo haploid induction system from diploid maize to hexaploid wheat

作者：Chenxu Liu, Yu Zhong, Xiaolong Qi, Ming Chen, Zongkai Liu, Chen Chen, Xiaolong Tian, Jinlong Li, Yanyan Jiao, Dong Wang, Yuwen Wang, Mengran Li, Mingming Xin, Wenxin Liu, Weiwei Jin, Shaojiang Chen · 发表于：Plant Biotechnology Journal · 年份：2019 · DOI：10.1111/pbi.13218 · 被引用次数：145 · 研究领域：CRISPR and Genetic Engineering、Chromosomal and Genetic Variations、Plant tissue culture and regeneration

Doubled haploid (DH) technology substantially accelerates crop breeding process. Wheat haploid production through interspecific hybridization requires embryo rescue and is dependent on genetic background. In vivo haploid induction (HI) in maize has been widely used and demonstrated to be independent of genetic background and to produce haploids efficiently. Recent studies revealed that loss-of-function of the gene MTL/ZmPLA1/NLD triggers HI (Gilles et al., 2017; Kelliher et al., 2017; Liu et al., 2017). In addition to producing homozygous DH lines, HI system has also been used for gene editing in different genetic backgrounds without introducing the genome of the male parent (Kelliher et al., 2019; Wang et al., 2019). Importantly, HI system had been successfully applied to rice (Yao et al., 2018), making this method more promising. However, little is known whether it can be applied to polyploids. Extension of the maize HI to wheat would create a novel approach in producing haploids in both wheat and other polyploid crop species. In this study, full-length amino acid sequence encoded by MTL/ZmPLA1/NLD was used to do BLAST search for homologues genes in different crop species (www.gramene.com). Results showed that the gene is highly conserved among 19 species of Liliopsida. Wheat homologues genes sharing ~70%-80% amino acid sequence identity with maize. Wheat genome contains three homologues genes: TraesCS4A02G018100 (TaPLA-A), TraesCS4B02G286000 (TaPLA-B) and TraesCS4D02G28470...