Expression of class II molecules on intestinal epithelial cells in humans

作者：Lloyd Mayer, David J. Eisenhardt, Peter F. Salomon, William M. Bauer, Ronley Plous, Linda A. Piccinini · 发表于：Gastroenterology · 年份：1991 · DOI：10.5555/uri:pii:0016508591905756 · 被引用次数：80 · 研究领域：Immune Cell Function and Interaction、T-cell and B-cell Immunology、Reproductive System and Pregnancy

Abstract Expression of class II antigens on human intestinal epithelial cells was assessed using a sensitive avidin-biotin-peroxidase technique. HLA-DR was present predominantly in the normal small bowel with diminished but evident expression in the colon. HLA-DP staining was less prominent, and HLA-DQ was absent. In inflammatory bowel disease the expression of both HLA-DR and HLA-DP was increased, but that for HLA-DQ remained absent, suggesting an inherent defect in the ability of intestinal epithelial cells to express HLA-DQ. In related experiments, an interferon gamma-treated malignant epithelial cell line T84 also failed to stain for HLA-DQ and HLA-DP despite the presence of HLA-DR. Isolated RNAs for all three subclasses of HLA-D were detectable by slot-blot analysis, suggesting that the lack of HLA-DQ expression relates to posttran-scriptional defects in intestinal epithelium. These and other differences with conventional class II antigen-positive accessory cells (macrophages/B cells) may help to explain the unique properties of intestinal epithelial cells as antigen-presenting cells.