GmSnRK1.1, a Sucrose Non-fermenting-1(SNF1)-Related Protein Kinase, Promotes Soybean Resistance to Phytophthora sojae

作者：Le Wang, Huiyu Wang, Shengfu He, Fanshan Meng, Chuanzhong Zhang, Sujie Fan, Junjiang Wu, Shuzhen Zhang, Pengfei Xu · 发表于：Frontiers in Plant Science · 年份：2019 · DOI：10.3389/fpls.2019.00996 · 被引用次数：28 · 研究领域：Plant-Microbe Interactions and Immunity、Legume Nitrogen Fixing Symbiosis、Plant Pathogens and Resistance

Phytophthora root and stem rot, a destructive disease of soybean [Glycine max (L.) Merr.], is caused by the oomycete Phytophthora sojae. However, how the disease resistance mechanisms of soybean respond to P. sojae infection remains unclear. Previously, we showed that GmWRKY31, which interacts with a sucrose nonfermenting-1(SNF1)-related protein kinase (SnRK), enhances resistance to P. sojae in soybean. Here, we report that the membrane-localized SnRK GmSnRK1.1 is involved in the soybean host response to P. sojae. The overexpression of GmSnRK1.1 (GmSnRK1.1-OE) increased soybean resistance to P. sojae, and the RNA interference (RNAi)-mediated silencing of GmSnRK1.1 (GmSnRK1.1-R) reduced resistance to P. sojae. Moreover, the activities and transcript levels of the antioxidant enzymes superoxide dismutase and peroxidase were markedly higher in the GmSnRK1.1-OE transgenic soybean plants than in the wild type (WT), but were reduced in the GmSnRK1.1-R plants. Several isoflavonoid phytoalexins related genes GmPAL, GmIFR, Gm4CL and GmCHS were significantly higher in ‘Suinong 10’ and GmSnRK1.1-OE lines than these in ‘Dongnong 50’, and were significantly lower in GmSnRK1.1-R lines. In addition, the accumulation of salicylic acid (SA) and the expression level of the SA biosynthesis-related gene were significantly higher in the GmSnRK1.1-OE plants than in the WT and GmSnRK1.1-R plants. Moreover, SA biosynthesis inhibitor treated GmSnRK1.1-R lines plants displayed clearly increased pathog...