Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy

作者：Changsheng Wang, Shican Tang, Qilin Zhan, Qingqing Hou, Yan Zhao, Qiang Zhao, Qi Feng, Congcong Zhou, Danfeng Lyu, Lingling Cui, Yán Li, Jiashun Miao, Chuanrang Zhu, Yiqi Lu, Yongchun Wang, Ziqun Wang, Jingjie Zhu, Yingying Shangguan, Junyi Gong, Yang Shi-hua, Wuqi Wang, Jianfu Zhang, Huaan Xie, Xuehui Huang, Bin Han · 发表于：Nature Communications · 年份：2019 · DOI：10.1038/s41467-019-11017-y · 被引用次数：121 · 研究领域：Genetic Mapping and Diversity in Plants and Animals、Genomics and Phylogenetic Studies、Genetic and phenotypic traits in livestock

Abstract Hybrid rice breeding for exploiting hybrid vigor, heterosis, has greatly increased grain yield. However, the heterosis-related genes associated with rice grain production remain largely unknown, partly because comprehensive mapping of heterosis-related traits is still labor-intensive and time-consuming. Here, we present a quantitative trait locus (QTL) mapping method, GradedPool-Seq, for rapidly mapping QTLs by whole-genome sequencing of graded-pool samples from F 2 progeny via bulked-segregant analysis. We implement this method and map-based cloning to dissect the heterotic QTL GW3p6 from the female line. We then generate the near isogenic line NIL-FH676:: GW3p6 by introgressing the GW3p6 allele from the female line Guangzhan63-4S into the male inbred line Fuhui676. The NIL-FH676:: GW3p6 exhibits grain yield highly increased compared to Fuhui676. This study demonstrates that it may be possible to achieve a high level of grain production in inbred rice lines without the need to construct hybrids.