Carriage of carbapenemase‐ and extended‐spectrum cephalosporinase‐producing Escherichia coli and Klebsiella pneumoniae in humans and livestock in rural Cambodia; gender and age differences and detection of bla OXA‐48 in humans

作者：Clara Atterby, Kristina Osbjer, Viktoria Tepper, Elisabeth Rajala, Jorge Hernández, Sokerya Seng, Davun Holl, Jonas Bonnedahl, Stefan Börjesson, Ulf Magnusson, Josef D. Järhult · 发表于：Zoonoses and Public Health · 年份：2019 · DOI：10.1111/zph.12612 · 被引用次数：51 · 研究领域：Antibiotic Resistance in Bacteria、Pharmaceutical and Antibiotic Environmental Impacts、Tuberculosis Research and Epidemiology

Abstract Objectives This study investigates the frequency and characteristics of carbapenemase‐producing Escherichia coli/Klebsiella pneumoniae (CPE/K) and extended‐spectrum cephalosporinase‐producing E. coli/K. pneumoniae (ESCE/K) in healthy humans and livestock in rural Cambodia. Additionally, household practices as risk factors for faecal carriage of ESCE/K are identified. Methods Faecal samples were obtained from 307 humans and 285 livestock including large ruminants, pigs and poultry living in 100 households in rural Cambodia in 2011. Each household was interviewed, and multilevel logistic model determined associations between household practices/meat consumption and faecal carriage of ESCE/K. CPE and ESCE/K were detected and further screened for colistin resistance genes. Results CPE/K isolates harbouring bla OXA‐48 were identified in two humans. The community carriage of ESCE/K was 20% in humans and 23% in livestock. The same ESBL genes: bla CTX‐M‐15 , bla CTX‐M‐14 , bla CTX‐M‐27 , bl a CTX‐M‐55 , bla SHV‐2 , bla SHV‐12 , bla SHV‐28 ; AmpC genes: bla CMY‐2 , bla CMY‐42, bla DHA‐1 ; and colistin resistance genes: mcr‐1‐like and mcr‐3‐like were detected in humans and livestock. ESCE/K was frequently detected in women, young children, pigs and poultry, which are groups in close contact. The practice of burning or burying meat waste and not collecting animal manure indoors and outdoors daily were identified as risk factors for faecal carriage of ESCE/K. Conclusions Faecal ...