Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

作者：Glenn Robert Masson, John E. Burke, Natalie G. Ahn, Ganesh S. Anand, Christoph H. Borchers, Sébastien Brier, George M. Bou-Assaf, John R. Engen, S. Walter Englander, Johan H. Faber, Rachel A. Garlish, Patrick R. Griffin, Michael L. Gross, Miklós Guttman, Yoshitomo Hamuro, Albert J. R. Heck, Damian J. Houde, Roxana E. Iacob, Thomas J. D. Jørgensen, Igor A. Kaltashov, Judith Pollock Klinman, Lars Konermann, Petr Man, Leland C. Mayne, Bruce D. Pascal, Dana Reichmann, Mark J. Skehel, Joost Snijder, Timothy S. Strutzenberg, Eric S. Underbakke, Cornelia Wagner, Thomas E. Wales, Benjamin T. Walters, David D. Weis, Derek J. Wilson, Patrick L. Wintrode, Zhongqi Zhang, Jie Zheng, David C. Schriemer, Kasper Dyrberg Rand · 发表于：Nature Methods · 年份：2019 · DOI：10.1038/s41592-019-0459-y · 被引用次数：804 · 研究领域：Mass Spectrometry Techniques and Applications、Analytical chemistry methods development、Isotope Analysis in Ecology

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances. Members of the hydrogen deuterium exchange mass spectrometry (HDX-MS) community provide their ‘best practices’ recommendations for HDX-MS data collection, analysis and reporting.