Renal T cell infiltration occurs despite attenuation of development of hypertension with hydralazine in Envigo’s female Dahl rat maintained on a low-Na + diet

作者：Amrita V. Pai, Crystal A. West, Aline M. A. de Souza, Parnika S. Kadam, Emma J. Pollner, David West, Jia Li, Hong Ji, Xie Wu, Michelle J. Zhu, Chris Baylis, Kathryn Sandberg · 发表于：American Journal of Physiology-Renal Physiology · 年份：2019 · DOI：10.1152/ajprenal.00512.2018 · 被引用次数：10 · 研究领域：Sodium Intake and Health、Hormonal Regulation and Hypertension、Electrolyte and hormonal disorders

Many studies have suggested that renal T cell infiltration contributes to the pathogenesis of salt-sensitive hypertension. To investigate this mechanism further, we determined T cell profiles in the kidney and lymphoid tissues as a function of blood pressure in the female Envigo Dahl salt-sensitive (SS) rat maintained on low-Na + (LS) diet. Mean arterial pressure and heart rate were measured by telemetry in SS rats from 1 mo old (juvenile) to 4 mo old. Normotensive salt-resistant (SR) rats were included as controls. Frequencies of T helper (CD4 + ) cells were greater in the kidney, lymph nodes, and spleen in 4-mo-old hypertensive SS rats compared with normotensive SR animals and SS juvenile rats, suggesting that renal T cell infiltration contributes to hypertension in the SS rat on a LS diet. At 1.5 mo, half of the SS rats were treated with vehicle (Veh), and the rest received hydralazine (HDZ; 25 mg·kg −1 ·day −1 ) for 11 wk. HDZ impeded the development of hypertension compared with Veh-treated control rats [mean arterial pressure: 157 ± 4 mmHg in the Veh-treated group ( n = 6) vs. 133 ± 3 mmHg in the HDZ-treated group ( n = 7), P < 0.001] without impacting T helper cell frequencies in the tissues, suggesting that HDZ can overcome mechanisms of hypertension driven by renal T cell infiltration under the LS diet. Renal frequencies of CD4 + CD25 + and CD4 + CD25 + FoxP3 + regulatory T cells were significantly higher in 4-mo-old hypertensive rats compared with normotensive SR...