Probiotics modulate the microbiota–gut–brain axis and improve memory deficits in aged SAMP8 mice

作者：Xueqin Yang, Dongke Yu, Xue Li, Hui Li, Junrong Du · 发表于：Acta Pharmaceutica Sinica B · 年份：2019 · DOI：10.1016/j.apsb.2019.07.001 · 被引用次数：409 · 研究领域：Gut microbiota and health、Probiotics and Fermented Foods、Tryptophan and brain disorders

ProBiotic-4 is a probiotic preparation composed of Bifidobacterium lactis , Lactobacillus casei , Bifidobacterium bifidum , and Lactobacillus acidophilus . This study aims to investigate the effects of ProBiotic-4 on the microbiota–gut–brain axis and cognitive deficits , and to explore the underlying molecular mechanism using senescence-accelerated mouse prone 8 (SAMP8) mice. ProBiotic-4 was orally administered to 9-month-old SAMP8 mice for 12 weeks. We observed that ProBiotic-4 significantly improved the memory deficits , cerebral neuronal and synaptic injuries , glial activation, and microbiota composition in the feces and brains of aged SAMP8 mice. ProBiotic-4 substantially attenuated aging-related disruption of the intestinal barrier and blood–brain barrier, decreased interleukin-6 and tumor necrosis factor- α at both mRNA and protein levels, reduced plasma and cerebral lipopolysaccharide (LPS) concentration, toll-like receptor 4 (TLR4) expression, and nuclear factor- κ B (NF- κ B) nuclear translocation in the brain. In addition, not only did ProBiotic-4 significantly decreased the levels of γ -H2AX, 8-hydroxydesoxyguanosine, and retinoic-acid-inducible gene-I (RIG-I), it also abrogated RIG-I multimerization in the brain. These findings suggest that targeting gut microbiota with probiotics may have a therapeutic potential for the deficits of the microbiota–gut–brain axis and cognitive function in aging, and that its mechanism is associated with inhibition of both TLR4-and...