PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation

作者：Duy-Chi Trinh, Julien Lavenus, Tatsuaki Goh, Yohann Boutté, Quentin Drogue, Virginie Vaissayre, Frédérique Tellier, Mikaël Lucas, Ute Voß, Pascal Gantet, Jean‐Denis Faure, Stéphane Dussert, Hidehiro Fukaki, Malcolm J. Bennett, Laurent Laplaze, Soazig Guyomarc’h · 发表于：Proceedings of the National Academy of Sciences · 年份：2019 · DOI：10.1073/pnas.1906300116 · 被引用次数：81 · 研究领域：Plant Molecular Biology Research、Lipid metabolism and biosynthesis、Plant nutrient uptake and metabolism

Lateral root organogenesis plays an essential role in elaborating plant root system architecture. In Arabidopsis , the AP2 family transcription factor PUCHI controls cell proliferation in lateral root primordia. To identify potential targets of PUCHI, we analyzed a time course transcriptomic dataset of lateral root formation. We report that multiple genes coding for very long chain fatty acid (VLCFA) biosynthesis enzymes are induced during lateral root development in a PUCHI-dependent manner. Significantly, several mutants perturbed in VLCFA biosynthesis show similar lateral root developmental defects as puchi-1 . Moreover, puchi-1 roots display the same disorganized callus formation phenotype as VLCFA biosynthesis-deficient mutants when grown on auxin-rich callus-inducing medium. Lipidomic profiling of puchi-1 roots revealed reduced VLCFA content compared with WT. We conclude that PUCHI-regulated VLCFA biosynthesis is part of a pathway controlling cell proliferation during lateral root and callus formation.