Bacterial Density and Biofilm Structure Determined by Optical Coherence Tomography

作者：Jiapeng Hou, Can Wang, René T. Rozenbaum, Niar Gusnaniar, E. de Jong, Willem Woudstra, Gésinda I. Geertsema-Doornbusch, Jelly Atema‐Smit, Jelmer Sjollema, Yijin Ren, Henk J. Busscher, Henny C. van der Mei · 发表于：Scientific Reports · 年份：2019 · DOI：10.1038/s41598-019-46196-7 · 被引用次数：74 · 研究领域：Bacterial biofilms and quorum sensing、Bacterial Identification and Susceptibility Testing、Optical Coherence Tomography Applications

Optical-coherence-tomography (OCT) is a non-destructive tool for biofilm imaging, not requiring staining, and used to measure biofilm thickness and putative comparison of biofilm structure based on signal intensity distributions in OCT-images. Quantitative comparison of biofilm signal intensities in OCT-images, is difficult due to the auto-scaling applied in OCT-instruments to ensure optimal quality of individual images. Here, we developed a method to eliminate the influence of auto-scaling in order to allow quantitative comparison of biofilm densities in different images. Auto- and re-scaled signal intensities could be qualitatively interpreted in line with biofilm characteristics for single and multi-species biofilms of different strains and species (cocci and rod-shaped organisms), demonstrating qualitative validity of auto- and re-scaling analyses. However, specific features of pseudomonas and oral multi-species biofilms were more prominently expressed after re-scaling. Quantitative validation was obtained by relating average auto- and re-scaled signal intensities across biofilm images with volumetric-bacterial-densities in biofilms, independently obtained using enumeration of bacterial numbers per unit biofilm volume. The signal intensities in auto-scaled biofilm images did not significantly relate with volumetric-bacterial-densities, whereas re-scaled intensities in images of biofilms of widely different strains and species increased linearly with independently determin...