Transcriptomic and metabolomic profiling of drought-tolerant and susceptible sesame genotypes in response to drought stress

作者：Jun You, Yujuan Zhang, Aili Liu, Donghua Li, Xiao Wang, Komivi Dossa, Rong Zhou, Jingyin Yu, Yanxin Zhang, Linhai Wang, Xiurong Zhang · 发表于：BMC Plant Biology · 年份：2019 · DOI：10.1186/s12870-019-1880-1 · 被引用次数：279 · 研究领域：Sesame and Sesamin Research、Peanut Plant Research Studies、Nitrogen and Sulfur Effects on Brassica

BACKGROUND: Sesame is an important oil crop due to its high oil, antioxidant, and protein content. Drought stress is a major abiotic stress that affects sesame production as well as the quality of sesame seed. To reveal the adaptive mechanism of sesame in response to water deficient conditions, transcriptomic and metabolomics were applied in drought-tolerant (DT) and drought-susceptible (DS) sesame genotypes. RESULTS: Transcriptomic analysis reveals a set of core drought-responsive genes (684 up-regulated and 1346 down-regulated) in sesame that was robustly differently expressed in both genotypes. Most enriched drought-responsive genes are mainly involved in protein processing in endoplasmic reticulum, plant hormone signal transduction photosynthesis, lipid metabolism, and amino acid metabolism. Drought-susceptible genotype was more disturbed by drought stress at both transcriptional and metabolic levels, since more drought-responsive genes/metabolites were identified in DS. Drought-responsive genes associated with stress response, amino acid metabolism, and reactive oxygen species scavenging were more enriched or activated in DT. According to the partial least-squares discriminate analysis, the most important metabolites which were accumulated under drought stress in both genotypes includes ABA, amino acids, and organic acids. Especially, higher levels of ABA, proline, arginine, lysine, aromatic and branched chain amino acids, GABA, saccharopine, 2-aminoadipate, and allantoi...