Effect of exogenous gonadotropin on the transcriptome of human granulosa cells and follicular fluid hormone profiles

作者：Cuiling Lu, Zhiqiang Yan, Xueling Song, Yang-Ying Xu, Xiaoying Zheng, Rong Li, Ping Liu, Huai-Liang Feng, Jie Qiao · 发表于：Reproductive Biology and Endocrinology · 年份：2019 · DOI：10.1186/s12958-019-0489-4 · 被引用次数：33 · 研究领域：Reproductive Biology and Fertility、Ovarian function and disorders、Reproductive System and Pregnancy

BACKGROUND: Superovulation treatment had some adverse effects on maturity and development of oocytes. Can superovulation dose of gonadotropins (Gns) affect the transcriptome of granulosa cells and follicular fluid (FF) hormone levels? METHODS: One leading pre-ovulatory follicle per subject was used from three natural-cycle and four Gn-stimulated patients. Granulosa cells and FF samples were collected from the same leading follicle of each patient. RNA was extracted from granulosa cells and subjected to deep sequencing and analysis. Follicle-stimulating hormone (FSH), estradiol (E2), androstenedione (AND), testosterone (T), luteinizing hormone (LH), and progesterone (P4) levels in FF were measured by immunoassays. Student's t test was used for statistical analysis. RESULTS: A total of 715 genes were up-regulated, and 287 genes were down-regulated, in the Gn-stimulated group relative to the control group. Gene Ontology analysis revealed that the down-regulated genes were enriched in cell cycle and meiosis pathways, primarily those associated with follicle or oocyte maturation and quality. On the other hand, the up-regulated genes were enriched in functions related to immunity and cytokine-cytokine receptor interactions. Compared to the follicles of natural cycle, the E2 and LH concentrations were significantly reduced (P < 0.001), the P4 concentration was significantly increased (P = 0.003), and the concentrations of FSH, T and AND had no difference in the follicles of Gn-stimu...