Circular RNAs negatively regulate cancer stem cells by physically binding FMRP against CCAR1 complex in hepatocellular carcinoma

作者：Yanjing Zhu, Bo Zheng, Guijuan Luo, Xu-Kai Ma, Xinyuan Lu, Ximeng Lin, Shuai Yang, Qing Zhao, Tong Wu, Zhixuan Li, Xiaolong Liu, Rui Wu, Jingfeng Liu, Yang Ge, Li Yang, Hongyang Wang, Lei Chen · 发表于：Theranostics · 年份：2019 · DOI：10.7150/thno.32796 · 被引用次数：328 · 研究领域：Circular RNAs in diseases、MicroRNA in disease regulation、Cancer-related molecular mechanisms research

Circular RNA (circRNA) possesses great pre-clinical diagnostic and therapeutic potentials in multiple cancers. It has been reported playing roles in multiple malignant behaviors including proliferation, migration, metastasis and chemoresistance. However, the underlying correlation between circRNAs and cancer stem cells (CSCs) has not been reported yet. Methods: circZKSCAN1 level was detected in HCC tissue microarrays to clarify its prognostic values. Gain and loss function experiments were applied to investigate the role of circZKSCAN1 in HCC stemness. Bioinformatic analysis was used to predict the possible downstream RNA binding protein and further RNA immunoprecipitation sequencing was carried out to identify the RBP-regulated genes.