<p>Decreased expression of SPINT1-AS1 and SPINT1 mRNA might be independent unfavorable prognostic indicators in esophageal squamous cell carcinoma</p>

作者：Fangfang Shen, Ying Pan, Haijun Yang, Jun-Kuo Li, Fanghui Zhao, Jing‐Fen Su, Yanyan Li, Linqiang Tian, Pan-Ting Yu, Yantian Cao, Yaowen Zhang, Fuyou Zhou · 发表于：OncoTargets and Therapy · 年份：2019 · DOI：10.2147/ott.s206448 · 被引用次数：26 · 研究领域：Cancer-related molecular mechanisms research、RNA modifications and cancer、Circular RNAs in diseases

Purpose: The serine peptidase inhibitor, Kunitz type 1 antisense RNA1 ( SPINT1-AS1 ), a long non-coding RNA , has been linked to cancer progression. In this study, we aimed to explore the SPINT1-AS1 expression in matched esophageal squamous cell carcinoma (ESCC) and normal tissues, and analyze the potential correlations of SPINT1-AS1 expression with clinicopathological characteristics, in order to evaluate its prognosis and therapeutic value. Methods: SPINT1-AS1 expression was detected in 99 cases of matched ESCC and normal tissues samples using the quantitative real-time polymerase chain reaction method. Results: The expression level (△Ct) of SPINT1-AS1 and SPINT1 mRNA was significantly downregulated in ESCC tissues compared with matched normal tissues ( P =0.0005; P =0.0002, respectively), and there was an obvious positive correlation between SPINT1-AS1 and SPINT1 mRNA expression. Clinicopathological characteristics indicated that SPINT1-AS1 expression was correlated with age and tumor size, while SPINT1 mRNA expression was correlated with age and gender. The receiver operating characteristic (ROC) curve analysis of the expression level of SPINT1-AS1 and SPINT1 mRNA yielded an area under the ROC curve value of 0.638 and 0.625, respectively. The overall survival is shorter in patients with low SPINT1-AS1 expressed than those with high levels of SPINT1-AS1 ( P =0.044), and SPINT1 mRNA expression level is associated with the OS ( P =0.001). Univariate and multivariate analysis...