Addition of pomegranate juice ( Punica granatum ) in tris‐based extender improves post‐thaw quality, motion dynamics and in vivo fertility of Nili Ravi buffalo ( Bubalus bubalis ) bull spermatozoa

作者：Moazam Javed, Muhammad Tarique Tunio, Hafiz Abdul Rauf, Muhammad Fahad Bhutta, Sadia Naz, Sajid Iqbal · 发表于：Andrologia · 年份：2019 · DOI：10.1111/and.13322 · 被引用次数：16 · 研究领域：Sperm and Testicular Function、Reproductive Biology and Fertility、Ovarian function and disorders

The aim of the present study was to determine the protective effects of pomegranate juice in tris-based extender on semen parameters, computer-assisted sperm analysis (CASA) motion characteristics and field fertility of post-thawed Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa. Two consecutive ejaculates/collection from each of the five adult Nili Ravi buffalo bulls were collected with artificial vagina at 42°C for a period of 7 weeks, diluted in extender containing different concentrations of pomegranate juice (0.0%, 2.5%, 5%, 7.5% and 10%). Diluted samples were packed and frozen in 0.54 ml French straws. The addition of 10% pomegranate juice in extender significantly improved post-thaw sperm morphology (%), motilities (CASA total motility, progressive motility (%) as well as VAP, VSL, VCL, STR, DAP, DSL) compared to the control group (p < 0.05). Plasma membrane, acrosome membrane and DNA integrity were significantly higher in extender with 10% pomegranate juice than the control group (p < 0.05). Field fertility rate (60.39% vs. 46.53%) was higher (p < 0.05) in extender with 10% pomegranate juice as compared to the control. It is therefore concluded that the addition of 10% pomegranate juice in tris-based extender improves post-thaw semen parameters, CASA motion dynamics and field fertility in Nili Ravi buffaloes.