UBN2 promotes tumor progression via the Ras/MAPK pathway and predicts poor prognosis in colorectal cancer

作者：Yali Zhao, Shenrong Zhong, Shihong Zhang, Jiaxin Bi, Zhiyuan Xiao, Shuyang Wang, Hong‐Li Jiao, Dan Zhang, Junfeng Qiu, Lingjie Zhang, Chengmei Huang, Xiaoling Chen, Yanqing Ding, Yaping Ye, Liang Li, Wenting Liao · 发表于：Cancer Cell International · 年份：2019 · DOI：10.1186/s12935-019-0848-4 · 被引用次数：20 · 研究领域：Ubiquitin and proteasome pathways、Genomic variations and chromosomal abnormalities、Cancer-related Molecular Pathways

BACKGROUND: Ubinuclein-2 (UBN2) is a nuclear protein that interacts with many transcription factors. The molecular role and mechanism of UBN2 in the development and progression of cancers, including colorectal cancer (CRC), is not well understood. The current study explored the role of UBN2 in the development and progression CRC. METHODS: Oncomine network and The Cancer Genome Atlas (TCGA) database were downloaded and Gene Set Enrichment Analysis (GSEA) was performed to compare the UBN2's expression between normal and tumor tissues, as well as the potential correlation of UBN2 expression with signaling pathways. Immunohistochemistry (IHC), qRT-PCR and Western blotting were performed to determine the expression of UBN2 in CRC tissues or cell lines. In vitro proliferation and invasion assays, and orthotopic mouse metastatic model were used to analyze the effect of UBN2 on the development and progression of CRC. RESULTS: The analysis of UBN2 expression using Oncomine network showed that UBN2 was upregulated in CRC tissues compared to matched adjacent normal intestinal epithelial tissues. IHC, qRT-PCR and Western blotting confirmed that UBN2 expression is higher in CRC tissues compared with matched adjacent normal intestinal epithelial tissues. In addition, analyses of TCGA data revealed that high UBN2 expression was associated with advanced stages of lymph node metastasis, distant metastasis, and short survival time in CRC patients. IHC showed that high UBN2 expression is correl...