Performance of the T2Bacteria Panel for Diagnosing Bloodstream Infections

作者：M. Hong Nguyen, Cornelius J. Clancy, A. William Pasculle, Peter G. Pappas, George Alangaden, George A. Pankey, Bryan H. Schmitt, Altaf Rasool, Melvin P. Weinstein, Raymond Widen, Diana R. Hernandez, Donna M. Wolk, Thomas J. Walsh, John R. Perfect, Mollie Wilson, Eleftherios Mylonakis · 发表于：Annals of Internal Medicine · 年份：2019 · DOI：10.7326/m18-2772 · 被引用次数：121 · 研究领域：Bacterial Identification and Susceptibility Testing、Neonatal and Maternal Infections、Streptococcal Infections and Treatments

Background: Blood cultures, the gold standard for diagnosing bloodstream infections (BSIs), are insensitive and limited by prolonged time to results. The T2Bacteria Panel (T2 Biosystems) is a direct-from-blood, nonculture test that identifies the most common ESKAPE bacteria (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Escherichia coli). Objective: To assess performance of the T2Bacteria Panel in diagnosing suspected BSIs in adults. Design: Prospective patient enrollment (8 December 2015 through 4 August 2017). Setting: Eleven U.S. hospitals. Patients: 1427 patients for whom blood cultures were ordered as standard of care. Intervention: Paired blood culture and T2Bacteria testing. Measurements: Performance of T2Bacteria compared with a single set of blood cultures in diagnosing proven, probable, and possible BSIs caused by T2Bacteria-targeted organisms. Results: Blood culture and T2Bacteria results were positive for targeted bacteria in 3% (39 of 1427) and 13% (181 of 1427) of patients, respectively. Mean times from start of blood culture incubation to positivity and species identification were 38.5 (SD, 32.8) and 71.7 (SD, 39.3) hours, respectively. Mean times to species identification with T2Bacteria were 3.61 (SD, 0.2) to 7.70 (SD, 1.38) hours, depending on the number of samples tested. Per-patient sensitivity and specificity of T2Bacteria for proven BSIs were 90% (95% CI, 76% to 96%) and 90% (CI, 88% to 91%), respectively...