Magnetic immobilisation of phospholipase C and its hydrolysis of phospholipids in crude soybean oil

作者：Dezhuang Yu, Chenxu Yu, Yina Jiang, X. Zhang, Tangju Yuan, Lifeng Wang, Walid Elfalleh, Lianzhou Jiang · 发表于：Quality Assurance and Safety of Crops & Foods · 年份：2019 · DOI：10.3920/qas2018.1321 · 被引用次数：2 · 研究领域：Enzyme Catalysis and Immobilization、Lipid metabolism and biosynthesis、Microbial Metabolic Engineering and Bioproduction

In this study, free phospholipase C (PLC) was immobilised onto magnetic carriers, including sodium alginate (MPCSA), magnetic chitosan microparticles (MCM), Fe3O4/P (glycidyl methacrylate (GMA)-EDGM-St) and Fe3O4/SiOx-g-P (GMA). The magnetic immobilised PLC had a broader pH activity and temperature tolerance, which could be separated quickly and reused. The highest enzyme loading and enzymatic activity (135.64 mg/g and 8,560 U/g, respectively) were obtained using the magnetic immobilised PLC-Fe3O4/SiOx-g-P (GMA) system. Its optimum pH range was 5.5-7.0, and activity remained stable between 50 and 70 °C. After 6 cycles in soybean lecithin emulsion, the magnetic immobilised enzyme retained more than 80% of its initial activity. Enzymatic degumming with PLC-Fe3O4/SiOx-g-P (GMA) was carried out 2.0 h at 60 °C, resulting in 1,2-diacylglycerol (1,2-DAG) content of 1.07%, which improving the refining rate of soybean oil. Moreover, PLC-Fe3O4/SiOx-g-P (GMA) still possessed more than 80% of its initial activity after 5 cycles of degumming in crude soybean oil. The results show that magnetic immobilised PLC has a good industrial application.