Detection of the phenicol–oxazolidinone–tetracycline resistance gene poxtA in Enterococcus faecium and Enterococcus faecalis of food-producing animal origin in China

作者：Changwei Lei, Zhuang-Zhuang Kang, Shun-Kang Wu, Yanpeng Chen, Linghan Kong, Hong-Ning Wang · 发表于：Journal of Antimicrobial Chemotherapy · 年份：2019 · DOI：10.1093/jac/dkz198 · 被引用次数：48 · 研究领域：Antimicrobial Resistance in Staphylococcus、Mycobacterium research and diagnosis、Antibiotic Resistance in Bacteria

Sir, Oxazolidinones, including linezolid and tedizolid, are effective antimicrobial agents for treating infections caused by MDR Gram-positive bacteria, including VRE.1,2 Linezolid is the first commercially available oxazolidinone that can inhibit protein synthesis by binding to the peptidyltransferase centre of the bacterial 23S rRNA. After introduction of linezolid, the resistance mechanism that emerged rapidly was related to mutations in genes coding for the 23S rRNA. The transferable oxazolidinone resistance determinants, cfr and optrA, have been reported in enterococci in several regions worldwide.3–6 Very recently, another transferable oxazolidinone resistance gene, poxtA, was reported in an MRSA of clinical origin and a porcine Enterococcus faecium isolate in Italy.7,8,poxtA encodes a ribosomal protection protein of the ABC-F family showing 32% identity to OptrA, which can decrease susceptibility to phenicols (chloramphenicol and florfenicol), oxazolidinones and tetracyclines.7 Here we evaluate the prevalence of poxtA in E. faecium and Enterococcus faecalis of food-producing animal origin in China. A total of 208 E. faecium (122 from swine and 86 from chicken) and 137 E. faecalis (79 from swine and 58 from chicken) strains were isolated from 360 faecal swabs (swine, n = 210; chicken, n = 150), which were collected from 36 food-producing animal farms in 12 provinces of China in 2017 (Table S1, available as Supplementary data at JAC Online). All strains were screened for...