[Protective effect of curcumin on dopamine neurons in Parkinson's disease and its mechanism].

作者：You Wu, Shunli Liang, Bin Xu, Rongbo Zhang, Linsheng Xu · 发表于：PubMed · 年份：2018 · DOI：10.3785/j.issn.1008-9292.2018.10.06 · 被引用次数：10 · 研究领域：Curcumin's Biomedical Applications、Magnolia and Illicium research、Parkinson's Disease Mechanisms and Treatments

OBJECTIVE: To investigate the effect of curcumin on dopamine neurons in Parkinson's disease (PD) and its mechanism. METHODS: SH-SY5Y human neuroblastoma cells were treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to establish the PD cell model. The model cells were treated with curcumin and/or autophagy inhibitor 3-MA. After 48 h of drug treatment, the number of surviving dopamine neurons was detected by tyrosine hydroxylase immunofluorescence method. Western blotting was used to detect protein expression of α-Synuclein (α-Syn), transcription factor EB (TFEB) and autophagy-related proteins lysosome-associated membrane protein 2A (LAMP2A) and microtubule-associated protein 1 light chain 3-Ⅱ(LC3-Ⅱ); RT-PCR was used to detect mRNA expression of α-Syn. RESULTS: <0.05). CONCLUSIONS: Curcumin exerts protective effect on dopamine neurons in PD, which may be associated with enhancing autophagy and promoting the clearance of α-Syn.