Development of a new monoclonal antibody specific to mouse Vγ6 chain

作者：Shinya Hatano, Xin Tun, Naoto Noguchi, Dan Yue, Hisakata Yamada, Xun Sun, Mitsuru Matsumoto, Yasunobu Yoshikai · 发表于：Life Science Alliance · 年份：2019 · DOI：10.26508/lsa.201900363 · 被引用次数：28 · 研究领域：T-cell and B-cell Immunology、Immune Cell Function and Interaction、Monoclonal and Polyclonal Antibodies Research

There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These hybridomas produced mAbs capable of cell surface staining of Vγ6/Vδ1 gene–transfected T-cell line lacking TCR as well as of Vγ1 − Vγ4 − Vγ5 − Vγ7 − γδ T cells and the CD3 high TCRδ int γδ T cells in various organs. The location of Vγ6 + γδ T cells, which peaked in the newborn thymus, was associated with mTEC. In vivo administration of clone 1C10-1F7 mAb impaired protection against Klebsiella pneumoniae infection but ameliorated psoriasis-like dermatitis induced by imiquimod treatment. These new mAbs are useful to elucidate the development, location, and functions of Vγ6 γδ T cells in mice.