Selective tetraspan–integrin complexes (CD81/α4β1, CD151/α3β1, CD151/α6β1) under conditions disrupting tetraspan interactions

作者：Valérie Serru, LE NAOUR François, Martine Billard, David O. Azorsa, François Lanza, Claude Boucheix, Eric P. Rubinstein · 发表于：Biochemical Journal · 年份：1999 · DOI：10.1042/bj3400103 · 被引用次数：172 · 研究领域：Cell Adhesion Molecules Research、Platelet Disorders and Treatments、Blood properties and coagulation

The tetraspans are molecules with four transmembrane domains which are engaged in multimolecular complexes (the tetraspan web) containing a subset of β1 integrins (in particular α3β1, α4β1 and α6β1), MHC antigens and several unidentified molecules. The molecules associated with tetraspans are readily detected after immunoprecipitation performed in mild detergents such as Brij 97 or CHAPS. In this study we show that another classical mild detergent, digitonin, dissociated most of these associated molecules, including integrins, from the tetraspans CD9, CD37, CD53, CD63, CD82, Co-029, Talla-1 and NAG-2. In contrast, reciprocal immunoprecipitations from various cell lines demonstrated that two other tetraspans, CD81 and CD151, formed complexes with integrins not disrupted by digitonin. These complexes were CD81/α4β1, CD151/α3β1 and CD151/α6β1. Furthermore, a new anti-CD151 monoclonal antibody (mAb), TS151r, was shown to have a restricted pattern of expression, inversely related to the sum of the levels of expression of α6β1 and α3β1. This mAb was unable to co-precipitate integrins in digitonin, suggesting that its epitope is blocked by the association with integrins. Indeed, the binding of TS151r to the cell surface was quantitatively diminished following α3β1 overexpression. Altogether, these data suggest that, among tetraspans, CD81 interacts directly with the integrin α4β1, and CD151 interacts directly with integrins α3β1 and α6β1. Because all tetraspan-tetraspan associations...