P2Y 14 receptor is functionally expressed in satellite glial cells and mediates interleukin‐1β and chemokine CCL2 secretion

作者：Jiu Lin, Fei Liu, Yan‐yan Zhang, Ning Song, Meng‐ke Liu, Xinyi Fang, Daqing Liao, Cheng Zhou, Hang Wang, Jiefei Shen · 发表于：Journal of Cellular Physiology · 年份：2019 · DOI：10.1002/jcp.28726 · 被引用次数：50 · 研究领域：Adenosine and Purinergic Signaling、Neuropeptides and Animal Physiology、Neuroinflammation and Neurodegeneration Mechanisms

Abstract Satellite glial cells (SGCs) activation in the trigeminal ganglia (TG) is critical in various abnormal orofacial sensation in nerve injury and inflammatory conditions. SGCs express several subtypes of P2 purinergic receptors contributing to the initiation and maintenance of neuropathic pain. The P2Y 14 receptor, a G‐protein‐coupled receptor activated by uridine diphosphate (UDP)‐glucose and other UDP sugars, mediates various physiologic events such as immune, inflammation, and pain. However, the expression, distribution, and function of P2Y 14 receptor in SGCs remains largely unexplored. Our study reported the expression and functional identification of P2Y 14 receptor in SGCs. SGCs were isolated from TG of rat, and the P2Y 14 receptor expression was examined using immunofluorescence technique. Cell proliferation and viability were examined via cell counting kit‐8 experiment. Immunofluorescence demonstrated the presence of P2Y 14 receptor in SGCs. Immunofluorescence and western blot showed that UDP‐glucose treatment upregulated glial fibrillary acid protein, a common marker for glial activation. Extracellular UDP‐glucose enhanced the phosphorylation of extracellular signal‐regulated kinase (ERK), c‐Jun N‐terminal kinase (JNK), and p38, which were both abolished by the P2Y 14 receptor inhibitor (PPTN). Furthermore, quantitative reverse transcription polymerase chain reaction and enzyme‐linked immunosorbent assay demonstrated that extracellular UDP‐glucose significantl...