Molecular changes in peripheral blood involving osteoarthritic joint remodelling

作者：Hongyun Zhang, Qian Liu, Jinqiang Liu, Jing Wang, Hongxu Yang, Xiaojie Xu, Mianjiao Xie, Xiaodong Liu, Shibin Yu, Mian Zhang, Lei Lü, Jing Zhang, Meiqing Wang · 发表于：Journal of Oral Rehabilitation · 年份：2019 · DOI：10.1111/joor.12810 · 被引用次数：10 · 研究领域：Temporomandibular Joint Disorders、Osteoarthritis Treatment and Mechanisms、Periodontal Regeneration and Treatments

Biomarkers of temporomandibular joint (TMJ) osteoarthritis (OA) remain unknown. The objective was to detect whether molecular biomarkers from peripheral blood leucocytes (PBLs) engage in TMJ OA lesions. Thirty-four six-week-old Sprague Dawley rats were used. The top upregulated gene ontology categories and gene-fold changes in PBLs were detected by a microarray analysis comparing rats that received 20-week unilateral anterior crossbite (UAC) treatment with age-matched controls (n = 4). Twenty weeks of UAC treatment had been reported to induce TMJ OA-like lesions. The other twenty-four rats were randomly placed in the UAC and control groups at 12- and 20-week time points (n = 6). The mRNA expression levels of the selected biomarkers derived from the microarray analysis and their protein expression in the alveolar bone and TMJ were detected. The microarray analysis indicated that the three most highly involved genes in PBLs were Egr1, Ephx1 and Il10, which were confirmed by real-time PCR detection. The increased protein expression levels of the three detected molecules were demonstrated in cartilage and subchondral bone (P < 0.05), and increased levels of EPHX1 were reported in discs (P < 0.05); however, increased levels were not present in the alveolar bone. Immunohistochemistry revealed the increased distribution of EGR1-positive, EXPH1-positive and IL10-positive cells predominantly in the osteochondral interface, with EXPH1 also present in TMJ discs. In conclusion, the incre...