Light-sheet microscopy with isotropic, sub-micron resolution and solvent-independent large-scale imaging

作者：Tonmoy Chakraborty, Meghan Driscoll, Malea M. Murphy, Philippe Roudot, Bo-Jui Chang, Saumya Vora, Wen Mai Wong, Cara D. Nielson, Hua Zhang, Vladimir Zhemkov, Chitkale Hiremath, Estanislao Daniel De La Cruz, Ilya Bezprozvanny, Hu Zhao, Raju Tomer, Rainer Heintzmann, Julian P. Meeks, Denise K. Marciano, Sean J. Morrison, Gaudenz Danuser, Kevin M. Dean, Reto Fiolka · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2019 · DOI：10.1101/605493 · 被引用次数：6 · 研究领域：Advanced Fluorescence Microscopy Techniques、Photoacoustic and Ultrasonic Imaging、Optical Coherence Tomography Applications

Abstract We present cleared tissue Axially Swept Light-Sheet Microscopy (ctASLM), which achieves sub-micron isotropic resolution, high optical sectioning capability, and large field of view imaging (870×870 μm 2 ) over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and organic chemically cleared tissue preparations and provides 2- to 5-fold better axial resolution than confocal or other reported cleared tissue light-sheet microscopes. We image millimeter-sized tissues with sub-micron 3D resolution, which enabled us to perform automated detection of cells and subcellular features such as dendritic spines.