Conversion of DON to 3- epi -DON in vitro and toxicity reduction of DON in vivo by Lactobacillus rhamnosus

作者：Rui Qu, Chunmei Jiang, Wanqin Wu, Bing Pang, Shuzhen Lei, Ziyang Lian, Dongyan Shao, Mingliang Jin, Junling Shi · 发表于：Food & Function · 年份：2019 · DOI：10.1039/c9fo00234k · 被引用次数：54 · 研究领域：Mycotoxins in Agriculture and Food、Potato Plant Research、Carcinogens and Genotoxicity Assessment

Deoxynivalenol (DON) is a mycotoxin that contaminates grains and feed. Degradation and toxicity reduction of DON by probiotics benefit human and animal health. Lactobacillus rhamnosus is an FDA-approved probiotic that can be used in children. After screening seven L. rhamnosus strains isolated from human milk, SHA113 showed the highest DON degradation rate of 60% under the optimal conditions of 37 °C, pH 6.0, OD600 = 1.5, 5 mg L-1 DON, and 48 h. When dead and live SHA113 cells were used separately, only the live cells reduced the DON concentration and transformed it into 3-epi-DON. Mice feeding experiments showed that pretreatment with SHA113 for 48 h reduced the toxicity of DON to the immunological system and organs. Directly feeding SHA113 cells could also slightly reduce the DON toxicity. Both in vitro and in vivo experiments indicated that L. rhamnosus has potential to reduce DON toxicity.