Epidermal growth factor‐containing fibulin‐like extracellular matrix protein 1 ( EFEMP 1) suppressed the growth of hepatocellular carcinoma cells by promoting Semaphorin 3B( SEMA 3B)

作者：Jiangfeng Hu, Bensong Duan, Weiliang Jiang, Sengwang Fu, Hengjun Gao, Lungen Lu · 发表于：Cancer Medicine · 年份：2019 · DOI：10.1002/cam4.2144 · 被引用次数：46 · 研究领域：Axon Guidance and Neuronal Signaling、Connective tissue disorders research、TGF-β signaling in diseases

AIM: Epidermal growth factor-containing fibulin-like extracellular matrix protein 1(EFEMP1) has been found to be involved in the occurrence and development of many cancers. The relationship between EFEMP1 and the development of hepatocellular carcinoma (HCC) and the molecular mechanism are not fully understood. METHODS: Real-time polymerase chain reaction (PCR) and tissue microarray were used to detect the expression of EFEMP1 in HCC cell lines and tissue. Methylation-specific PCR assay was used to measure the methylation level of EFEMP1 in HCC cell lines and tissue. To study the function of EFEMP1 on cell function, Huh7 and HepG2 were infected with lentiviral particles expressing EFEMP1. MTT assay and colony formation assay were used to examine the effect of EFEMP1 on cell proliferation. Annexin-VAPC/7-AAD double were used to detect the effect of EFEMP1 on cell apoptosis. To further detect the effect of EFEMP1 on the development of HCC in vivo, we performed the tumor formation experiment in nude mice. Gene chip was used to detect the expression profile of Huh7 and HepG2 overexpressing EFEMP1. To further screen out the differences, GO analysis and pathway analysis were performed. To study the effects of SEMA3B, specific siRNA was used to inhibit the expression of SEMA3B. Chi-squared test and rank sum test were used to analyze the relationship between EFEMP1 expression and HCC clinical characteristic. RESULTS: The study found that the expression of EFEMP1 was significantly dec...